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. 1994 Mar;1(2):247–249. doi: 10.1128/cdli.1.2.247-249.1994

Demonstration of human immunoglobulin G Fc-binding activity in oral bacteria.

D Grenier 1, J Michaud 1
PMCID: PMC368238  PMID: 7496956

Abstract

Nonimmune binding of immunoglobulins via the Fc fragment may reduce opsonization and phagocytosis of bacteria and is thus considered a virulence factor. The aim of this study was to investigate a wide range of oral bacterial strains for the presence of human immunoglobulin G (IgG) Fc-binding activity. A total of 132 strains representing 40 different gram-positive and gram negative bacterial species were tested for IgG Fc-binding activity by using a fast and simple dot blot procedure with horseradish peroxidase-conjugated Fc fragments from human IgG. Neither the human nor animal biotype of Porphyromonas gingivalis possessed IgG Fc-binding activity. The strongest positive reaction of gram-negative species with the IgG Fc fragments were obtained with strains of Prevotella intermedia and Fusobacterium nucleatum. Among the gram-positive bacteria tested, Peptostreptococcus micros, Lactobacillus spp., and several species of streptococci possessed IgG Fc-binding activity. In the present investigation, the ability of several oral bacterial species to bind IgG Fc fragments was demonstrated. This factor represents a potential virulence determinant as it may help pathogenic oral bacteria escape host defense mechanisms.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dossett J. H., Kronvall G., Williams R. C., Jr, Quie P. G. Antiphagocytic effects of staphylococcal protein A. J Immunol. 1969 Dec;103(6):1405–1410. [PubMed] [Google Scholar]
  2. Dzink J. L., Socransky S. S., Haffajee A. D. The predominant cultivable microbiota of active and inactive lesions of destructive periodontal diseases. J Clin Periodontol. 1988 May;15(5):316–323. doi: 10.1111/j.1600-051x.1988.tb01590.x. [DOI] [PubMed] [Google Scholar]
  3. Forsgren A., Sjöquist J. "Protein A" from S. aureus. I. Pseudo-immune reaction with human gamma-globulin. J Immunol. 1966 Dec;97(6):822–827. [PubMed] [Google Scholar]
  4. Hardie J. M. Oral microbiology: current concepts in the microbiology of dental caries and periodontal disease. Br Dent J. 1992 Apr 11;172(7):271–278. doi: 10.1038/sj.bdj.4807849. [DOI] [PubMed] [Google Scholar]
  5. Kronvall G. A surface component in group A, C, and G streptococci with non-immune reactivity for immunoglobulin G. J Immunol. 1973 Nov;111(5):1401–1406. [PubMed] [Google Scholar]
  6. Lamster I. B., Celenti R., Ebersole J. L. The relationship of serum IgG antibody titers to periodontal pathogens to indicators of the host response in crevicular fluid. J Clin Periodontol. 1990 Aug;17(7 Pt 1):419–425. doi: 10.1111/j.1600-051x.1990.tb02340.x. [DOI] [PubMed] [Google Scholar]
  7. Lebrun L., Pillot J., Grangeot-Keros L., Rannou M. T. Detection of human Fc (gamma) receptors on streptococci by indirect immunofluorescence staining: a survey of streptococci freshly isolated from patients. J Clin Microbiol. 1982 Jul;16(1):200–201. doi: 10.1128/jcm.16.1.200-201.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moore W. E., Holdeman L. V., Cato E. P., Smibert R. M., Burmeister J. A., Palcanis K. G., Ranney R. R. Comparative bacteriology of juvenile periodontitis. Infect Immun. 1985 May;48(2):507–519. doi: 10.1128/iai.48.2.507-519.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Movitz J., Masuda S., Sjöquist J. Physico- and immunochemical properties of staphylococcal protein A extracellularly produced by a set of mutants from Staphylococcus aureus Cowan I. Microbiol Immunol. 1979;23(2):51–60. doi: 10.1111/j.1348-0421.1979.tb00441.x. [DOI] [PubMed] [Google Scholar]
  10. Myhre E. B., Erntell M. A non-immune interaction between the light chain of human immunoglobulin and a surface component of a Peptococcus magnus strain. Mol Immunol. 1985 Aug;22(8):879–885. doi: 10.1016/0161-5890(85)90073-2. [DOI] [PubMed] [Google Scholar]
  12. Peterson P. K., Verhoef J., Sabath L. D., Quie P. G. Effect of protein A on staphylococcal opsonization. Infect Immun. 1977 Mar;15(3):760–764. doi: 10.1128/iai.15.3.760-764.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Raeder R., Boyle M. D. Association between expression of immunoglobulin G-binding proteins by group A streptococci and virulence in a mouse skin infection model. Infect Immun. 1993 Apr;61(4):1378–1384. doi: 10.1128/iai.61.4.1378-1384.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Savitt E. D., Socransky S. S. Distribution of certain subgingival microbial species in selected periodontal conditions. J Periodontal Res. 1984 Mar;19(2):111–123. doi: 10.1111/j.1600-0765.1984.tb00800.x. [DOI] [PubMed] [Google Scholar]
  15. Tolo K., Helgeland K. Fc-binding components: a virulence factor in Actinobacillus actinomycetemcomitans? Oral Microbiol Immunol. 1991 Dec;6(6):373–377. doi: 10.1111/j.1399-302x.1991.tb00509.x. [DOI] [PubMed] [Google Scholar]
  16. Widders P. R., Dorrance L. A., Yarnall M., Corbeil L. B. Immunoglobulin-binding activity among pathogenic and carrier isolates of Haemophilus somnus. Infect Immun. 1989 Feb;57(2):639–642. doi: 10.1128/iai.57.2.639-642.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yarnall M., Widders P. R., Corbeil L. B. Isolation and characterization of Fc receptors from Haemophilus somnus. Scand J Immunol. 1988 Aug;28(2):129–137. doi: 10.1111/j.1365-3083.1988.tb02424.x. [DOI] [PubMed] [Google Scholar]

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