Figure 2.

Edema and aberrant fluid uptake in the skin of the adult Adm^AM haploinsufficient mice after hind limb skin incision wounding surgery. Wild-type (Adm^AM+/+) and Adm^AM haploinsufficient mice without (Adm^AM+/Δ) or with exogenous AM supplement (Adm^{AM+/Δ suppl}) were used for surgery. (a) The incision site (black arrow) and the ankle (white arrowhead) are indicated (bar=5 mm). For comparison, the images of the sham-operated limbs are presented in Supplementary Figure S3a online. (b) Hind limb swelling degree was assessed and compared (Wilcoxon rank test) between groups. (c, d) Evans blue uptake by inguinal lymph nodes was assessed (four mice in each experimental group) and compared (t-test). Representative images of the inguinal lymph nodes (white arrowheads) after dye uptake are shown (bar=1.5 mm).