Table 1.
Antibodies used in this study
| Primary antibody (catalog #) | Source of primary; dilution | Secondary antibody | Source of secondary; dilution |
|---|---|---|---|
| Pab VGlut2 (AB5907) | Millipore; 1:2500 | Goat anti-guinea pig-488 | Invitrogen; 1:1000 |
| DAPI | Invitrogen; 1:1000 | N/A | N/A |
| Draq5 | Biostatus, Ltd.; 1:1000 | N/A | N/A |
| DBA—biotin (B-1035) | Vector Laboratories; 1:50 | Avidin-568 | Vector Laboratories; 1:1000 |
| Pab Tnc (AB19013; lot #24010549) | Millipore; 1:200 (IF) 1:1000 (WB) | Donkey anti-rabbit-555 (IF) | Invitrogen; 1:1000 (IF) |
| Donkey anti-rabbit-HRP (WB) | Jackson ImmunoResearch; 1:1000 (WB) | ||
| Mab Tnc clone MTn-12 (T3413) | Sigma-Aldrich; 1:1000 | Donkey anti-rat-HRP (WB) | Jackson ImmunoResearch; 1:1000 (WB) |
| Mab β-actin (AC-15) | Abcam; 1:1000 | Goat anti-mouse IgG-HRP | Jackson ImmunoResearch; 1:1000 |
| Pab NCAM (AB5032) | Millipore; 1:1000 | Donkey anti-rabbit-555 | Invitrogen; 1:1000 |
| Mab phosphacan (MAB5210) | Millipore; 1:500 | Goat anti-mouse IgM-555 | Invitrogen; 1:1000 |
Abbreviations: IF, Immunofluoresence; WB, Western blot; Pab, polyclonal antibody; Mab, monoclonal antibody.