Skip to main content
. Author manuscript; available in PMC: 2013 Jun 14.
Published in final edited form as: Lab Invest. 2010 Apr 19;90(6):812–823. doi: 10.1038/labinvest.2010.73

Figure 4.

Figure 4

Subgroup analysis of peripheral blood monocyte subpopulations and fibrogenic mediators in young vs. aged controls. A: Staining with anti-CD45-PE vs. intracellular isotype control antibody used to set negative gates. B:CD45+/pro-Col-1α+ staining (CD45-PE vs. pro-Col-Iα) in young healthy control. C: Anti-CD45-PE vs intracellular isotype control antibody used to set negative gates. D: CD45+/pro-Col-1α+ staining (CD45-PE vs. pro-Col-Iα) in aged healthy control. E: Fibrocyte percentages (left axis) and quantities (right axis) in young subjects (white bar), aged subjects (black bar) and SSc-ILD patients (slanted lines). F: Comparison of lymphocytes (dotted line), monocytes (checked bar) and CD45+/pro-Col-Iα+ cells (black bar) in (left to right) young, aged, and SSc-ILD subjects. G: CD14+ monocyte expression of CD163 after 48 hours in culture. H-O: Concentrations of the plasma cytokines IL-4 (H), IL-10 (I), IL-13 (J), IL-1 RA (K), MCP-1 (L), TNFα (M), CCL-18 (N) and M-CSF (O) in young controls (n = 10, white bar), aged controls ( n = 12, black bar) and patients with SSc-ILD (n = 11, slanted bar). All data are expressed as mean ± SEM. *p<0.05. **p<0.01. ***p<0.001.

HHS Vulnerability Disclosure