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. Author manuscript; available in PMC: 2013 Jun 14.
Published in final edited form as: Eur J Endocrinol. 2011 Nov 2;166(1):69–75. doi: 10.1530/EJE-11-0571

Figure 5.

Figure 5

Functional studies. (A) The six lanes on the left correspond to experiments done with a labeled oligonucleotide specific for allele C of the rs2816948 polymorphism, whereas in the six lanes on the right a labeled oligonucleotide specific for allele G was used. NE indicates that no extract was added to the labeled probe. In lanes labeled C and G, nuclear extracts were added to labeled C- and G-specific probes respectively. In lanes labeled 10×, 25×, and 50×, a 10-, 25-, and 50-fold excess of an unlabeled C-specific oligonucleotide was used for interfering the formation of the complexes by either C-specific or G-specific probes. In the lanes labeled as NS, a 50-fold excess of a nonspecific probe was used for competition. The specific complex is indicated with an arrow. (B) Differences in the transcriptional activities of C- and G-specific fragments when cloned in a luciferase reporter vector. The results are the average of four different experiments (***P<0.005).