Fig. 2.
In vitro cultivation of Fascioloides magna (Fasiciolidae) miracidia in untreated CBSS (Chernin’s balanced salt solution; Chernin, 1963). Within 24 h, most miracidia cease swimming activity but the majority will not fully transform (i.e. will retain their ciliated epidermal plates (A). However, miracidia cultured in CBSS, pre-conditioned by cultivation with snail Biomphalaria glabrata embryonic (Bge) cells, are induced to transform (rounding and shedding of ciliated epidermal plates (B) to become fully-transformed primary sporocysts (C).