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. Author manuscript; available in PMC: 2013 Jun 14.
Published in final edited form as: Int J Parasitol. 2008 Oct 21;39(5):533–539. doi: 10.1016/j.ijpara.2008.09.011

TABLE 1.

Effects of mutations in E. coli CPS or Hamster CPSII

Mutation Location in Tg Effect on CPS or CPSII
Ec C269A Tg C345 Abolishes GATase activity
Ec G359F Tg G435 Ammonia leaks, uncoupling GATase & CPS
Ha T456A Tg T533 Abolishes MAPK activation
Ec E761A Tg E1316 Abolishes ornithine activation and UMP repression
Ec H781K Tg H1336 Reduces CPS activity, ornithine activation, and UMP repression
Ec T974A Tg T1530 Reduces ornithine activation, IMP activation, and UMP repression
Ha S1345A Tg T1530 Reduces PRPP activation
Ec T1042A Tg T1649 Reduces ornithine binding

Escherichia coli (Ec); Hamster (Ha); T. gondii (Tg)

See text for references and Supplemental Figure for alignments. Table 1 identifies the location of residues in T. gondii CPSII that correspond to residues previously examined in mutagenesis studies in either E. coli or Hamster CPS enzymes.