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. Author manuscript; available in PMC: 2013 Jun 14.
Published in final edited form as: Int J Parasitol. 2008 Oct 21;39(5):533–539. doi: 10.1016/j.ijpara.2008.09.011

TABLE 3.

Effect of CPS mutations on T. gondii CPSII complementation activity

CPS Mutation growth rate (h) transient efficiency
(% of control vacuoles)		 stable efficiency
(% of control PFU)
Tg Pc 4 (w.t.) 7.4 ± 0.08 100 100
Tg T533A 7.4 ± 0.18 105 ± 7.2 98 ± 6.9
Tg S581A 7.3 ± 0.14 109 ± 11 104 ± 8.5
Tg Δ873-910 8.2 ± 0.27 113 ± 12 65 ± 11
Tg E1316A nd 0 0
Tg E1318A nd 0 0
Tg H1336K nd 0 0
Tg T1430A 7.7 ± 0.21 86 ± 5.7 82 ± 8.4
Tg T1530A 8.6 ± 0.25 51 ± 13 10 ± 3.2
Tg T1530 fs nd 0 0
Tg Δ1592-1628 nd 0 0
Tg T1649A 8.5 ± 0.21 65 ± 5.2 37 ± 7.0

no growth rate detected (nd). Table 3 shows the effect of engineered mutations in the CPS domains of T. gondii CPSII on parasite growth rate, transient and stable complementation efficiency in cps1-1 complementation assays. See Table 2 for descriptions of complementation.