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. Author manuscript; available in PMC: 2014 May 10.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Apr 10;434(3):439–443. doi: 10.1016/j.bbrc.2013.03.092

Figure 3.

Figure 3

Effects of vinpocetine on LOX-1 expression in vitro and in vivo. (A) Vinpocetine inhibits ox-LDL-induced LOX-1 expression in macrophages. RAW264.7 cells were starved in 0.1% FBS-starved for 24 h, pretreated with indicated doses of vinpocetine for 1 h, followed by treatment with 50 μg/ml ox-LDL for 24 h, the mRNA levels were measured by real time RT-PCR. Values are means ± SD from at least three independent experiments. *P<0.05 vs. ox-LDL with zero vinpocetine. (B–E) Vinpocetine inhibits LOX-1 expression in vivo. Representative images (B) stained with Oil-red O (left panels), LOX-1 antibody (middle panels), or macrophage marker Mac-2 (right panels), in aortic sinus of ApoE knockout mice fed with a high-fat diet (HF) for 16 weeks with i.p injection of 5 mg/kg/day vinpocetine (Vinp) or vehicle (Veh). (C–E) Quantitative data of Oil-red O, LOX-1 and MAC-2 positive areas. Values are means ± SEM from at least five animals. *P<0.05 compared with saline i.p injection. Magnification: ×100.