Figure 4.
Replication of the 5′-T-(MeFapy-Gua)-T-3′ (2a, top) and unmodified control (2b, bottom) templates by hRev1, the combined activities of hRev1 and yPol ζ with the −1 primer, and extension by yPol ζ with the 0 primer. The dNTP concentrations are for dCTP alone for the hRev1 insertion and for each dNTP for the full-length extension reactions. Results for the 5′-TXG-3′ (1) sequence are part of the Supporting Information (Figure S3).