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. Author manuscript; available in PMC: 2013 Jun 14.
Published in final edited form as: Clin Cancer Res. 2010 Jan 12;16(2):474–485. doi: 10.1158/1078-0432.CCR-09-1322

Figure 2. HER2 protein expression on primary GBM.

Figure 2

(A) FACS analysis: primary GBM cells from freshly excised tumors in short term culture were stained for HER2 expression (isotype control: open curves; HER2: solid curve. Nine out of ten tumor cell lines expressed HER2 on the cell surface. (B) Using the HER2-specific mouse monoclonal antibody NCL-L-CB11 (NovocastraTM Newcastle upon Tyne, UK), HER2 expression was confirmed on the corresponding paraffin-embedded sections. One tumor had no detectable HER2 protein expression using both methods (patient 8). Magnification 200x.