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. 2013 Apr 30;288(24):17074–17081. doi: 10.1074/jbc.R113.473108

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Effect of purification on compound I preparation. See text for a discussion of the EP (purified) and HP (highly purified) labels. A, spectrum of EP-CYP119A1 (black) and a spectrum taken (at maximum formation of P450-I) during the reaction of EP-CYP119A1 with 2 eq of m-CPBA at 4 °C (blue). Compound I is formed in very low yield. B, spectrum of HP-CYP119A1 (black) and a spectrum taken (at maximum formation of P450-I) during the reaction of HP-CYP119A1 with 2 eq of m-CPBA at 4 °C (blue). Compound I can be produced in up to ∼75% yield. C, overlay of HP-CYP119A1 (red) and EP-CYP119A1 (black).