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. 2013 Apr 26;288(24):17111–17121. doi: 10.1074/jbc.M112.447326

FIGURE 1.

FIGURE 1.

Construction of RII variant phage library. A, structural representation of low-variability aliphatic residues on one face of the AKAP anchoring helix (orange) and the reciprocal binding surface in the RII D/D domain (residues 1–45; gray). B, residues in PKA RII that contact AKAP positions of higher variability (orange spheres). C, frequencies of each amino acid at every position in the first 20 amino acids of RII expressed on the surface of the phage in the input library. D, distribution of mutant nucleotides within the RII coding sequences of the input RII phage library. Mutations in wild-type RII coding sequence were restricted to codons 3, 5, 10, and 14. E, distribution of single, double, triple, and quadruple RII mutants in the input phage library. F, high-throughput sequencing statistics of RII variant phage libraries. Rd., round; n/a, not applicable.