TABLE 1.
Dissociation rate constants (10−6 s−1)/association-rate constants (106m−1s−1) for BLIP-II alanine scanning mutants
| BLIP-II variantc |
koff/kon |
|||
|---|---|---|---|---|
| TEM-1 | Bla1 | KPC-2 | CTX-M-14 | |
| WT | 3.7/7.7 | 0.6/1.9 | 0.8/10.1b | 1.7/3.2 |
| N50A | 19/4.4 | 19.6/1.4 | 1.1/9.3 | 9.9/1.9 |
| D52A | 23.6/3.9 | 14.2/1.1 | 9.5/10.1 | 126.2/2.4 |
| W53A | 15.6/3.1 | 47.2/1.3 | 696.3/8.6 | 3961/4.1a |
| T57A | 6/7.7 | 0.7/2.2 | 1.2/15 | 5.3/4.2 |
| Y73A | 564.6/5.8 | 65.7/1.6 | 223.5/21.2 | 501.9/3.1 |
| F74A | 184.3/5.2 | 425.9/1.7 | 514.3/11.2 | 2649/3.3a |
| L91A | 8.4/5.7 | 1.9/1.6 | 6/11.1 | 62/3.4 |
| N112A | 20.1/5.4 | 5.6/1.3 | 5.8/12.3 | 31.5/2.2 |
| Y113A | 9.5/8.8 | 2.7/2.3 | 2.2/20.3 | 8.6/4 |
| D131A | 79.2/5.7 | 15.2/2.1 | 2.3/12.7 | 75.3/3.8 |
| W152A | 404.4/3.5 | 151/0.8 | 61.2/9.1 | 720.8/2.6a |
| D167A | 19.2/7.9 | 1.4/2.4 | 2/16.6 | 7.3/4.7 |
| S169A | 3.4/5.5 | 0.5/2 | 0.8/13.7 | 1.9/2.8 |
| D170A | 1.5/8 | 2.4/1.6 | 2.1/3.4 | 2.3/6.7 |
| Y191A | 533.8/4.3 | 162.2/1.7 | 48.2/11.3b | 160.3/1.7 |
| D206A | 1.1/13.7 | 215.3/1.9 | 0.5/5 | 1/5.3 |
| Y208A | 105.9/7.2 | 7.5/1.3 | 3.1/12 | 12.6/3 |
| F209A | 91/4.6 | 14/1.4 | 1.7/9.7 | 6.4/3.1 |
| I229A | 341.2/5.4 | 96.7/1.8 | 32.9/11.9 | 165.8/3.1 |
| F230A | 818/2.1 | 86.8/1.6 | 6/7.6 | 22.2/1.8 |
| Y248A | 17.9/9.2 | 1/2.3 | 9.9/14.8 | 7.8/4.7 |
| E268A | 103.9/9.5 | 105.3/2.2 | 51.3/11.2 | 108.9/5.2 |
| W269A | 38180/4.4a | 3168/1.1 | 3223/2.5a | 360.1/1.7a |
| R286A | 20/9.3 | 1.2/1.6 | 0.4/16.7 | 2.2/3.7 |
| N304A | 239.1/5.5 | 41.5/1.5 | 12.7/11 | 205/2.7 |
a Indicates that the association rate constant was determined using the bimolecular binding differential equation. The remaining constants were determined by the second order association rate equation.
b The data for BLIP-II wild-type and BLIP-II Y191A interactions with KPC-2 from Ref. 39.
c All of the determined association and dissociation rate constants have an associated standard error that is <20%.