TABLE 2.

Dissociation constants (pm) for BLIP-II alanine scanning mutants

BLIP-II variant	Kd
	TEM-1	Bla1	KPC-2	CTX-M-14
WT	0.48	0.30	0.08a	0.54
N50A	4.32	13.53	0.12	5.20
D52A	6.12	13.52	0.95	53.70
W53A	5.04	37.73	80.59	972.92
T57A	0.79	0.33	0.08	1.26
Y73A	98.11	40.33	10.54	161.20
F74A	35.63	249.94	46.08	810.87
L91A	1.48	1.19	0.53	18.01
N112A	3.70	4.51	0.47	14.61
Y113A	1.08	1.16	0.11	2.15
D131A	13.79	7.28	0.18	19.57
W152A	116.84	179.98	6.75	278.19
D167A	2.43	0.57	0.12	1.57
S169A	0.62	0.26	0.06	0.68
D170A	0.19	1.52	0.62	0.35
Y191A	125.54	97.01	4.26a	94.43
D206A	0.08	110.47	0.10	0.19
Y208A	14.71	5.69	0.26	4.22
F209A	19.71	9.75	0.17	2.05
I229A	63.08	53.18	2.76	53.15
F230A	384.94	53.85	0.79	12.61
Y248A	1.93	0.45	0.67	1.67
E268A	10.88	48.53	4.58	21.10
W269A	8738.51	2877.38	1296.95	218.25
R286A	2.15	0.74	0.03	0.61
N304A	43.54	28.37	1.15	76.49

^a Data for BLIP-II interaction with KPC-2 from Ref. 39.