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Stable expression of TRB3 does not affect A3A toxicity or localization. A, MTS assay for viability of three T-REx 293 A3A TRB3 stable clones as compared with vector stable clone and T-REx 293 A3A-E72A TRB3 and vector stable clones. Dox, doxycycline. Error bars indicate S.D. B, immunoblots of A3A (anti-A3A) and TRB3 (anti-FLAG) from the cells in panel D treated with 0 and 100 pg/ml doxycycline. HSP90 is a loading control.
