FIGURE 4.

Analysis of ceramide species following HU treatment in WT and isc1Δ cells by mass spectrometry. Phytoceramide levels were determined after 3 and 20 h of treatment with HU. To determine the fold changes of phytoceramide species due to HU, the values obtained from the treated samples were divided by the untreated ones. Fold changes in phytoceramides are represented in A for WT, in B for isc1Δ after 3 h, in C for WT, and in D, for isc1Δ after 20 h. All measurements represent the mean of two independent experiments.