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. 2013 May 3;288(24):17384–17398. doi: 10.1074/jbc.M112.444042

FIGURE 4.

FIGURE 4.

Localization of Hog1 or transcription factors Hot1, Msn2, and Sko1 is not altered in NPC mutant strains. A, Hog1 localization was not affected in NPC mutant strains. WT and the indicated NPC mutant strains expressing Hog1-GFP were grown in SD medium to mid-log phase and subjected to osmostress (0.4 m NaCl, 5 min). DAPI staining to reveal nuclei and epifluorescence pictures were taken without (basal conditions) and with stress. The percentage of cells with Hog1 nuclear accumulation upon stress is shown in the lower panel; data are the mean and S.D. of three independent experiments. B, Hot1, Msn2, and Sko1 localization is not affected in NPC mutant strains. WT and the indicated NPC mutant strains expressing Hot1-GFP, Mns2-GFP, and Sko1-GFP were grown in SD medium to mid-log phase and subjected to osmostress (0.4 m NaCl, 5 min). Nomarsky and epifluorescence pictures were taken without (−) and with stress (+). DAPI staining to reveal nuclei in the wild type strain upon stress is shown in the lower panel.