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NPC localization is not affected upon osmostress. Yeast strains expressing Nup60-GFP, Nup1-GFP, or Nup2-GFP were grown in SD medium to mid-log phase and subjected to osmostress (0.4 m NaCl or 1.2 m NaCl, 5 min). Epifluorescence pictures were taken without (basal conditions) and with stress.
