FIGURE 7.

Activation of thrombolytic zymogens by KLK5 and KLK12. The increase in fluorescence (440 nm) of the GGGR-7-amino-4-methylcoumarin fluorogenic substrate was monitored upon treatment of plasminogen (A and B), prourokinase (C and D), and plasma prekallikrein (E and F) with kallikrein 5 (A, C, and E) and kallikrein 12 (B, D, and F). Each graph includes the change in fluorescence of zymogen alone (□), KLK5 and KLK12 alone (○), and zymogen with KLK5 and KLK12 (♢). RFU, relative fluorescence units.