FIGURE 4.

A, side chain interactions at the N terminus of huE06 v1.1 include a network of hydrogen bonds as well as hydrophobic interactions. Together these interactions may contribute to the presentation of the neighboring CDR3 and to the overall stability of the V-NAR. B, in the parental E06 shark sequence, the HV2 region features a network of hydrogen bonds that position Lys³⁹, Ser⁴⁴, and Lys⁴⁶ for interaction with HSA. Disruption of these networks would negatively impact antigen binding. C, the HV4 region may influence the positioning of Tyr³², which is directly involved in HSA binding.