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Retention of albumin binding by humanized E06 v1.1 and v1.10. A, sequence alignment of parental E06 with humanized E06 v1.1 and v1.10 with residues in E06 involved in the indicated modes of interactions with HSA marked by arrows. DPK9-like residues in E06 and huE06 are shown in bold. B, sandwich ELISA with purified E06 and huE06-hFc fusion proteins on human albumin. C, direct ELISA with purified monomeric E06 and huE06 on human and bovine albumin.
