TABLE 3.
Kinetic parameters of human, mouse, and rat albumin binding by E06 and its humanized variants at pH 7.4 and pH 6.0
n/a, not applicable.
| V-NAR | Albumin | pH | ka | kd | KD | -Fold change from E06 |
|---|---|---|---|---|---|---|
| 1/m s | 1/s | nm | ||||
| E06 | 3.48e+06 | 6.55e−04 | 0.19 | n/a | ||
| huE06 v1.1 | Human | 7.4 | 8.72e+05 | 1.40e−02 | 16.0 | 84.9 |
| huE06 v1.10 | 5.59e+05 | 3.57e−03 | 6.4 | 33.9 | ||
| E06 | 2.18e+06 | 1.80e−03 | 0.83 | n/a | ||
| huE06 v1.1 | Mouse | 7.4 | 8.41e+05 | 4.14e−02 | 49.2 | 59.4 |
| huE06 v1.10 | 4.12e+05 | 9.59e−03 | 23.2 | 28.1 | ||
| E06 | 2.21e+06 | 3.20e−03 | 1.45 | n/a | ||
| huE06 v1.1 | Rat | 7.4 | 4.36e+05 | 3.29e−02 | 75.3 | 52.0 |
| huE06 v1.10 | 4.49e+05 | 1.65e−02 | 36.8 | 25.4 | ||
| E06 | 1.12e+07 | 1.59e−03 | 0.14 | n/a | ||
| huE06 v1.1 | Human | 6.0 | 3.62e+06 | 1.84e−02 | 5.07 | 35.6 |
| huE06 v1.10 | 3.52e+06 | 6.37e−03 | 1.81 | 12.7 | ||
| E06 | 9.85e+06 | 3.23e−03 | 0.33 | n/a | ||
| huE06 v1.1 | Mouse | 6.0 | 3.43e+06 | 3.18e−02 | 9.38 | 28.3 |
| huE06 v1.10 | 3.14e+06 | 1.25e−02 | 3.98 | 12.2 |