TABLE 1.
Summary of pH responses from WT and mutant GLIC channels
Data are the mean ± S.E. from n experiments. pH50 is the pH value that elicited 50% of the maximal proton induced current (Imax). nH is the Hill coefficient.
| Channel | Location | pH50 | nH | Imax | n |
|---|---|---|---|---|---|
| μA | |||||
| WT | 5.1 ± 0.05 | 1.7 ± 0.07 | 13.0 ± 2.4 | 6 | |
| C26A | 5.2 ± 0.07 | 1.8 ± 0.12 | 12.8 ± 1.2 | 9 | |
| I201C (M1) | Intra | 4.6 ± 0.07a | 2.1 ± 0.25 | 5.0 ± 0.8a | 4 |
| N238C (M2-15′) | Inter | 4.4 ± 0.06a | 1.7 ± 0.13 | 4.3 ± 1.0a | 5 |
| L240C (M2-17′) | Inter | 4.6 ± 0.06a | 1.8 ± 0.04 | 9.1 ± 1.9 | 3 |
| V241C (M2-18′) | Intra | 4.3 ± 0.07a | 2.4 ± 0.28 | 1.7 ± 0.7a | 3 |
| E242C (M2-19′) | Inter | 5.0 ± 0.08 | 1.7 ± 0.14 | 10.9 ± 1.5 | 8 |
| T243C (M2-20′) | Pore | 4.6 ± 0.09a | 2.1 ± 0.09 | 4.5 ± 2.1a | 4 |
| T254C (M3) | Intra | 6.8 ± 0.03a | 3.1 ± 0.15a | 9.3 ± 1.2 | 4 |
a Values significantly different from WT; p < 0.01.