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. 2013 Apr 29;288(24):17460–17471. doi: 10.1074/jbc.M113.468405

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Metabolic responses to HAMLET, oleate, or oleic acid. Metabolites were quantified by GC/MS in extracts of A549 cells treated with HAMLET (35 μm) or oleate (175 μm) for 60 min. A, shown is a heatmap of differentially abundant metabolites. A global decrease in metabolite abundance was detected in the HAMLET samples. B, frequency distribution of log 2-transformed-fold changes in signal intensity between HAMLET-treated and oleate-treated cells is shown. C, targeted metabolite profiling is shown. Change in abundance of lipids, amino acids, and the citric acid cycle metabolites in HAMLET- or oleate-treated cells is shown. n.s., not significant.