Cav3 mutants affect Kir2. 1 current density by significantly reducing surface expression of Kir2.1 channels.
A, representative on-cell Western blot analysis illustrating intensity levels of extracellular HA-tagged Kir2.1 surface expression in wells cotransfected with HA-Kir2.1 and vector only (b), WT-Cav3 (c), F97C-Cav3 (d), and P104L-Cav3 (e). a shows untreated cells stained with both primary and secondary antibody. B, the graph displays quantitative results of three independent experiments.