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. 2013 May 2;288(24):17472–17480. doi: 10.1074/jbc.M112.435370

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Cav3 mutants affect Kir2. 1 current density by significantly reducing surface expression of Kir2.1 channels. A, representative on-cell Western blot analysis illustrating intensity levels of extracellular HA-tagged Kir2.1 surface expression in wells cotransfected with HA-Kir2.1 and vector only (b), WT-Cav3 (c), F97C-Cav3 (d), and P104L-Cav3 (e). a shows untreated cells stained with both primary and secondary antibody. B, the graph displays quantitative results of three independent experiments.