Alignment of Ifh1 protein sequences across the ascomycetes species highlighting the conserved and partially conserved regions. The FHB domain is the only extended stretch to show conservation across all species, whereas a smaller region is seen to be conserved across those Ifh1 proteins binding with Rap1; this region is missing in those species where Tbf1 is responsible for ribosomal protein transcriptional control in place of Rap1. Darker color indicates greater conservation. RB, Rap1 binding region; C.alb, C. albicans; S.cer, S. cerevisiae; S.par, Saccharomyces paradoxus; C.gla, Candida glabrata; S.cas, Saccharomyces castellii; A.gos, Ashbya gossypii; K.lac, Kluyveromyces lactis; K.wal, Kluyveromyces waltii; L.elo, Lodderomyces elongisporus; C.par, Candida parapsilosis; D.han, Debaryomyces hansenii; C.lus, Candida lusitaniae; C.gui, Candida guilliermondii; C.tro, Candida tropicalis; Y.lip, Yarrowia lipolytica; S.klu, Saccharomyces kluyveri.