FIGURE 3.

Tbf1 immunoprecipitates itself only if the TRB domain is intact. A, co-immunoprecipitations from lysates of the different C. albicans strains where one copy of Tbf1 is either full-length (FL) or C-terminally deleted with a Myc tag and the other copy of Tbf1 is full-length and C-terminally tagged with HA (details of strains used are shown in Table 1). B, the interaction of the TRB domain of Tbf1 with itself as observed in the yeast two-hybrid system. IP, immunoprecipitation; Ab, antibody; −ve, negative.