FIGURE 6.

A, immunoblot of different C. albicans strains with one copy of a Myc-tagged Tbf1, either full length (FL) or C-terminally deleted, and with the other full-length copy of Tbf1 tagged with HA and expressed under the Met3p promoter (details of strains used are in Table 1). The strains were grown in the presence or absence of methionine and cysteine. The presence of methionine and cysteine suppresses the expression of the HA-tagged full-length copy of Tbf1. B, the growth assay shows that in the absence of full-length Tbf1 the growth of the strains is severely compromised both in the absence and presence of stress-inducing factors. C shows the result of B in a tabular form. IP, immunoprecipitation; Ab, antibody; −ve, negative; ver., version.