FIGURE 4.

MLL5 protein does not associate with core component proteins ASH2L, RBBP5, and WDR5. A, HEK293T cells were transiently transfected with plasmids encoding 3× FLAG-tagged MLL5 protein or the C-180 fragment of the MLL protein. Expression of the MLL5 protein and MLL C-180 fragment were detected by using anti-FLAG antibody. Endogenous ASH2L, RBBP5, and WDR5 proteins were detected by Western blotting using polyclonal antibodies against ASH2L, RBBP5, and WDR5, respectively. B, the presence of ASH2L, RBBP5, WDR5, and OGT proteins were detected in the MLL C-180 fragment or MLL5 precipitates using polyclonal antibodies against ASH2L, RBBP5, WDR5, and OGT, respectively.