TABLE 1.
List of MLL5-associated proteins identified by mass spectrum
| Gene symbol | Hits | Molecular mass | Activity |
|---|---|---|---|
| HCF-1 | 26 | 213 kDa | Nuclear coactivator |
| XRCC6 | 12 | 70 kDa | Ku70 |
| DHX9 | 10 | 14 1kDa | Putative RNA helicases |
| XRCC5 | 9 | 83 kDa | Ku80 |
| DDX21 | 9 | 87 kDa | Putative RNA helicases |
| DDX3X | 8 | 73 kDa | Putative RNA helicases |
| ILF2 | 7 | 43 kDa | Interleukin enhancer-binding factor |
| HNRNPU | 7 | 90 kDa | RNA-binding protein |
| SYNCRIP(HNRNPQ) | 7 | 70 kDa | RNA-binding protein |
| SSB Lupus La protein | 7 | 49 kDa | Lupus La protein |
| HNRNPR | 6 | 71 kDa | RNA-binding protein |
| IGF2BP1 | 6 | 63 kDa | IGF-II mRNA-binding protein |
| OGTa | 5 | 117 kDa | O-Linked N-acetylglucosamine (O-GlcNAc) transferase |
| YBX1 | 5 | 36 kDa | YB1 |
| DDX5 | 5 | 69 kDa | Putative RNA helicases |
| HNRNPA1 | 5 | 39 kDa | RNA-binding protein |
| ILF3 | 4 | 96 kDa | Interleukin enhancer-binding factor |
| FOXK1 | 4 | 75 kDa | Forkhead box protein K1 |
| PARP1 | 4 | 113 kDa | Ribosyltransferase |
| HNRNPM | 4 | 76 kDa | RNA-binding protein |
| KPNB1 | 4 | 97 kDa | NLS import receptor |
| PABPC1 | 4 | 71 kDa | Poly(A)-binding protein |
| CSDA | 4 | 40 kDa | DNA-binding protein A |
| NPM1 | 3 | 32 kDa | Assembly and/or transport of ribosome |
| TRIM28 | 3 | 89 kDa | E3 sumo; ligase |
| DDX17 | 3 | 80 kDa | Putative RNA helicases |
| U2AF2 | 3 | 54 kDa | A non-snRNP protein |
| HNRNPF | 3 | 46 kDa | RNA binding protein |
| DDX1 | 3 | 80 kDa | Putative RNA helicases |
| HNRNPA2B1 | 3 | 28 kDa | RNA-binding protein |
| HNRNPAB | 3 | 36 kDa | RNA-binding protein |
| HNRNPD | 3 | 38 kDa | RNA-binding protein |
| NSUN2 | 3 | 86 kDa | RNA methyltransferase |
| IGF2BP3 | 3 | 64 kDa | mRNA-binding protein |
| SNRNP200 | 3 | 245 kDa | Putative RNA helicases |
| G3BP1 | 3 | 52 kDa | DNA-unwinding enzymes |
| DNAJA1 | 3 | 45 kDa | DnaJ (Hsp40)homolog subfamily A member |
a HCF-1 and OGT were confirmed by co-immunoprecipitation assay in our study and are in boldface type.