FIGURE 3.

Ser-862 phosphorylation of both mGluR7a and mGluR7b is regulated by PP1γ1. A, generation of mGluR7b-specific antibody is shown. HEK cells were transfected with vector (Vec-TF), mGluR7a (mGluR7a-TF), or mGluR7b (mGluR7b-TF). Two days after transfection, cells were solubilized and immunoblotted with mGluR7a or mGluR7b antibody, respectively. B, primary cortical neurons were treated with dimethyl sulfoxide (DMSO) or okadaic acid (50 nm) for 45 min. Neurons were washed three times and solubilized using 1% Triton X-100 lysis buffer and immunoprecipitated with mGluR7a or mGluR7b antibody. Immunoprecipitates were resolved by SDS-PAGE and immunoblotted using the indicated antibodies. WB, Western blot.