FIGURE 3.

MICU1 modulates ΔΨ_m responses in malignant cells. A and B, in A2780 cells loaded with the fluorescent ΔΨ_m indicator DiBASC₂(3), ⁺AuNPs caused rapid and substantial membrane depolarization (A), whereas nonmalignant OSE cells showed slight hyperpolarization (B). ⁺AuNP effects on ΔΨ_m in A2780 cells were enhanced by MICU1 blockade (siRNA). NT, nontreated controls. C–F, these ⁺AuNP effects on ΔΨ_m were also evident in confocal images of the fluorescent dye JC-1 in malignant cells (C) versus normal cells (D) and corresponding measurements of JC-1 (E and F). Inhibition of uniporter by Ru360 enhanced ⁺AuNP effects on ΔΨ_m. A similar effect was observed with MICU1 siRNA (not shown). Scale bar = 20 μm; n = 3. w/o ⁺AuNP, without ⁺AuNP.