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Detection of apoptotic cells in the retinas of transgenic mice with increased intraocular IGF-I. Cells positive for the apoptosis marker cleaved caspase 3 (green, arrows) were found in the ganglion cell layer of transgenic retinas at 6 months of age. Cleaved caspase 3-positive cells could not be detected in retinal sections from age-matched wild-type animals. Nuclei were stained with DAPI (blue). Scale bar, 7.6 μm (left panel), and 11.43 μm (right panel).
