Impaired retinal glial functionality in mice with increased intraocular IGF-I.
A, retinal GS activity was assayed in WT and TgIGF-I mice by spectrophotometric monitoring of γ-glutamyl hydroxamate. GS activity was significantly reduced in transgenic animals from an early age. Values are expressed as mean ± S.E. of 8–13 animals/group. *, p <0.05. B, follow up of retinal TNF-α expression by quantitative PCR. TNF-α was noticeably increased in TgIGF-I at 7.5 months of age. Values are expressed as mean ± S.E. of 4–5 animals/group. *, p <0.05. C, retinal MCP-1 content in 7.5 month-old WT and TgIGF-I mice analyzed by Western blot. MCP-1 levels were significantly higher in transgenic retinas. Values are expressed as mean ± S.E. of four animals/group. **, p <0.01. tub, tubulin.