FIGURE 4.

SENP1 rescues Sharp-1-mediated suppression of myogenesis. A, C2C12 cells were transfected with vector alone (control), Myc-Sharp-1, or Sharp-1 2KR individually or together with SENP1. Lysates were immunoblotted with anti-Myc antibody. B and C, after selection, differentiation was analyzed at day 2 with anti-MHC antibody. Nuclei were stained with DAPI (B). The myogenic index was determined. D, ChIP assays were performed to determine H3K9me2 enrichment at the myogenin promoter in cells expressing vector, Sharp-1, Sharp-1 2KR, and Sharp-1 along with SENP1. E and F, ChIP assay was performed to determine occupancy of G9a (E) and Sharp-1 (F) at the myogenin promoter in cells expressing vector alone, Sharp-1, and Sharp-1 2KR. Error bars indicate mean ± S.D.