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Hydrolysis of Schiff base in Meta II-like activated product, acid denaturation assay for activated WT rhodopsin, D190N, and S186W mutants. a, WT; b, D190N, and c, S186W rhodopsin at 37 °C (top) and 55 °C (bottom). Over time, A440 decreases, and A380 increases, indicating hydrolysis of SB. d, normalized A440 plotted as a function of time and fit to obtain the rate constant khydT.
