TABLE 1.
Rate constants and half-lives (± S.D.) of thermal processes of WT rhodopsin, D190N, and S186W mutants
All experiments were performed 3–5 times.
| Sample | Rate constants, min−1 (half-lives, min) |
Contribution of each pathway |
||||
|---|---|---|---|---|---|---|
| kisob | khydc | kiso | khydT | kisob | khydc | |
| % | % | |||||
| WT, 55 °C | 0.005 ± 0.001 (126 ± 26) | 0.004 ± 0.001 (182 ± 61) | 0.003 ± 0.002 (236 ± 188) | 0.888 ± 0.034 (0.781 ± 0.029) | 59 ± 16 | 41 ± 16 |
| D190N, 55 °C | 0.233 ± 0.022 (2.972 ± 0.283) | 0.115 ± 0.021 (6 ± 1) | 0.138 ± 0.051 (5 ± 2) | 0.751 ± 0.023 (0.923 ± 0.029) | 67 ± 9 | 33 ± 7 |
| S186W, 55 °C | 1.549 ± 0.043 (0.447 ± 0.013) | 0.251 ± 0.037 (2.762 ± 0.403) | 0.047 ± 0.023 (15 ± 7) | 0.929 ± 0.035 (0.746 ± 0.028) | 86 ± 4 | 14 ± 2 |
| S186W, 37 °C | 0.003 ± 0.001 (245 ± 44) | 0.017 ± 0.001 (41 ± 2) | 0.005 ± 0.001 (142 ± 8) | 0.153 ± 0.003 (4.532 ± 0.075) | 14 ± 3 | 86 ± 5 |