TABLE 2.
Rate constants and half-lives (± S.D.) of thermal processes of WT rhodopsin, D190N, and S186W mutants after accounting for the difference between ϵtrans and ϵcis
| Sample | Rate constants, min−1 (new model) |
Contribution of each pathway |
||||
|---|---|---|---|---|---|---|
| khydb | khydc | kiso | khydT | kisob | khydc | |
| % | % | |||||
| WT, 55 °C | 0.005 ± 0.001 (0.005 ± 0.001) | 0.004 ± 0.001 (0.004 ± 0.001) | 0.003 ± 0.002 (0.003 ± 0.002) | 0.888 ± 0.034 (0.888 ± 0.034) | 59 ± 16 (59 ± 16) | 41 ± 16 (41 ± 16) |
| D190N, 55 °C | 0.233 ± 0.022 (0.241 ± 0.021) | 0.115 ± 0.021 (0.100 ± 0.020) | 0.138 ± 0.051 (0.115 ± 0.050) | 0.751 ± 0.023 (0.751 ± 0.023) | 67 ± 9 (71 ± 9) | 33 ± 7 (29 ± 6) |
| S186W, 55 °C | 1.549 ± 0.043 (1.566 ± 0.045) | 0.251 ± 0.037 (0.213 ± 0.038) | 0.047 ± 0.023 (0.042 ± 0.025) | 0.929 ± 0.035 (0.929 ± 0.035) | 86 ± 4 (88 ± 4) | 14 ± 2 (12 ± 2) |
| S186W, 37 °C | 0.003 ± 0.001 (0.003 ± 0.001) | 0.017 ± 0.001 (0.017 ± 0.001) | 0.005 ± 0.001 (0.005 ± 0.001) | 0.153 ± 0.003 (0.153 ± 0.003) | 14 ± 3 (15 ± 3) | 86 ± 5 (85 ± 5) |