FIGURE 2.

NKp80 of human NK cells is phosphorylated, but fails to recruit Syk. A, NKp80 surface expression by NK cells expanded in co-cultures with RPMI8866 cells from PBL of two healthy donors. Expanded NK cells (gate: CD3⁻CD56⁺) were stained with mAb NKp80 (solid lines) or an isotype control (filled) and analyzed by flow cytometry. B, NKp80 from lysates of pervanadate (PV)-treated or untreated human NK cells was immunoprecipitated with anti-NKp80 mAb 5D12 and precipitates were analyzed for NKp80 (polyclonal anti-NKp80 reagent), tyrosine phosphorylation (pY), or presence of Syk by immunoblotting after removal of N-linked carbohydrates. Arrow indicates anticipated position of Syk. Immunoprecipitations (IP) with an isotype control IgG1 is shown for control.