FIGURE 6.

Syk association with NKp80 is detectable upon Syk overexpression. A, immunoblots of lysates of expanded, polyclonal NK cells, Jurkat cells, and NK-92MI transduced with wildtype or kinase-deficient (KD) mutants of Syk or ZAP70 (B) FACS plots of NK-92MI cells ectopically expressing NKp80 or NKp80 in conjunction with Syk (KD). Syk expression is indicated by co-expressed GFP. C, NKp80 was precipitated from lysates of untreated or pervanadate-treated NK-92MI cells ectopically expressing NKp80 alone or in conjunction with Syk KD using mAb 5D12. Precipitates were assayed for tyrosine phosphorylation and co-precipitated Syk by immunoblotting. NKp80 precipitation was monitored with a polyclonal anti-NKp80 reagent.