FIGURE 4.

Fsk treatment accelerates EF2K turnover in HEK cells. A, HEK cells were transfected with GFP-EF2K and treated with 10 or 50 μm Fsk in the presence of Chx (30 μg/ml) to examine the rate of EF2K degradation. GFP-EF2K and GAPDH levels in lysates were assessed by SDS-PAGE and immunoblot. B, graph of summary data. A two-way ANOVA revealed significant main effects of chase time and Fsk dose, as well as a significant interaction between time and dose. Tukey's post-hoc tests revealed that both 10 and 50 μm Fsk significantly decreased GFP-EF2K levels relative to control (n = 3, ** or ****, p < 0.01 or p < 0.0001, respectively).