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Cul1 regulates EF2K levels. A, HEK cells were transfected with GFP-EF2K with or without FLAG-DN-Cul1 and GFP-EF2K, FLAG-DN-Cul1, and GAPDH levels in lysates were assessed 48 h later by SDS-PAGE and immunoblot. B, graph of summary data. Co-expression of FLAG-DN-Cul1 significantly increased steady-state levels of GFP-EF2K to 982% ± 229 of cells expressing only GFP-EF2K as determined by t test, n = 4, *, p < 0.05.
