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βTRCP regulates EF2K levels. A, HEK cells were transfected with GFP-EF2K with or without sh-βTRCP and levels of GFP-EF2K and GAPDH in lysates were assessed by SDS-PAGE and immunoblot 48 h later. B, graph of summary data. A t test demonstrated that knock-down of βTRCP significantly increased steady-state GFP-EF2K levels to 1441% ± 276 of control, n = 3, *, p < 0.05.
