Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 May 10;288(24):17832–17843. doi: 10.1074/jbc.M112.448977

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 6. — Hbs1 and Dom34 interact with the exosome-Ski complex. HeLa cells were transfected with either p5×Myc-Ski2 (A) or p5×Myc-Dis3 (B) and also pFLAG (lanes 1 and 4), pFLAG-Hbs1 (lanes 2 and 5), or pFLAG-Dom34 (lanes 3 and 6). The cell extracts were subjected to an immunoprecipitation (IP) assay using anti-FLAG antibody. Immunoprecipitation was performed in the presence of RNase A. The immunoprecipitates (lanes 4–6) and inputs (lanes 1–3; 10% of the amount immunoprecipitated) were analyzed by Western blotting (WB) using the indicated antibodies. The results are representative of three independently performed experiments.