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. 2013 May 4;288(24):17871–17883. doi: 10.1074/jbc.M112.437756

FIGURE 2.

FIGURE 2.

Tyrosine phosphorylation of KAP1 at Tyr-449, Tyr-458, and Tyr-517. A, C, E, a schematic representation of KAP1. RING, RING finger domain; B box, B box zinc fingers 1 and 2; CC, coiled-coil domain; HP1 box, heterochromatin protein 1 box; PHD, plant homeodomain; Bromo, Bromo domain. B, D, and F–H, COS-1 cells cotransfected with NLS-Lyn plus the indicated plasmids were cultured for 24 h. KAP1-wt and its mutants were immunoprecipitated (IP) with anti-FLAG antibody. Immunoblotting (WB) was performed with the indicated antibodies. I, KAP1-wt or KAP1–3YF immunoprecipitates were incubated with Lyn immunoprecipitates, as described in the legend to Fig. 1G. Immunoblotting was performed with the indicated antibodies. J, COS-1 cells cotransfected with KAP1-wt plus NLS-Lyn or KAP1–3YF plus the indicated plasmids were cultured for 24 h. KAP1-wt and KAP1–3YF were immunoprecipitated with anti-FLAG antibody. Immunoblotting was performed with the indicated antibodies. Asterisks show degradation products.