FIGURE 5.

AKAP5 is required for association of ACs but not the β₂-AR with PSD. A and C, 10 μg of crude lysate (Lys), P2, synaptosome-enriched (Syn), and PSD fractions from WT (W) and either AKAP5 KO (K) mice (A) or D36 (D) mice (C) were separated by SDS-PAGE before immunoblotting for the indicated proteins. B and D, PSD signals for each protein from D36 (B) or KO mice (D) were quantified and normalized to the respective PSD-95 signal for each sample. C and E, AC5/6 signals in synaptosome-enriched and PSD fractions from WT and AKAP5 KO mice were quantified and compared with each other. The data represent the average ± S.E. (error bars) for n independent experiments (**, p < 0.01; ***, p < 0.001, t test).