AKAP5 is necessary for GluA1 Ser-845 phosphorylation upon β2-AR stimulation in the hippocampus.
A, acute hippocampal slices from 8–12-week-old WT C57BL/6 and AKAP5 KO mice were incubated with vehicle or ISO (10 μm) for 5 min before solubilization, IP of GluA1, immunoblotting with antibodies against phospho-Ser-845 (pS845), stripping, and reprobing for phospho-Ser-831 (pS831) and ultimately total GluA1 (GluA1). B and C, immunosignals were quantified for phospho-Ser-845 and phospho-Ser-831 and corrected for variations in total GluA1 content. Graphed are averages ± S.E. (error bars) of relative phosphorylation levels (*, p < 0.05, two-way ANOVA).