Figure 1.

Organization of the soybean ferritin transformation construct. The construct consisted of the super gamma zein promoter, the soybean ferritin coding sequence and the Tvsp terminator from soybean vegetative storage protein gene. The corresponding sizes for each construct component are indicated in brackets below the construct component name. The ATG and TAG indicate the start and stop sites, respectively, while NcoI and SacI represent the enzyme restriction sites flanking the soybean ferritin sequence with the plastid transit peptide. The forward and the reverse primer directions are indicated by arrows pointing in opposite directions.